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RG011,OD104,Pickering/皮克林柱后衍生氨基酸試劑

價(jià)格

￥1,680.00

型號(hào)

RG011,OD104

品牌

Pickering/皮克林

所在地

天津市南開區(qū)

更新時(shí)間

2020-08-26 12:40:04

瀏覽次數(shù)

次

產(chǎn)品描述

鈉離子交換柱法測(cè)氨基酸--
RG011,OD104,皮克林/Pickering柱后衍生

OPA 用于衍生分析氨基酸是1971年首次提出。圖 1 為 OPA 與一級(jí)氨基酸的反應(yīng)方程式。

室溫下， OPA （Pickering O120）在巰基乙醇(Pickering 3710-2000)環(huán)境中與一級(jí)氨基酸于 1 min 內(nèi)即可生成具有強(qiáng)熒光的異吲哚衍生產(chǎn)物。OPA 本身不發(fā)熒光的特性使其在色譜分離時(shí)不形成干擾峰。OPA 只能與經(jīng)次氯酸鈉等氧化劑氧化開環(huán)后的二級(jí)氨基酸反應(yīng)，也可與 FMOC － Cl 聯(lián)用衍生二級(jí)氨基酸。OPA 衍生法因樣品制備簡(jiǎn)單、衍生反應(yīng)迅速、靈敏度高及易于實(shí)現(xiàn)自動(dòng)化操作等優(yōu)點(diǎn)而成為柱前衍生反相高效液相色譜法中使用廣泛的方法。

OPA 衍生法（用于檢測(cè)一級(jí)氨基酸）

試劑： 300 mg OPA （Pickering O120）, 2 g Thiofluor? (Pickering 3710-2000), 3 mL 30 % Brij® 35 溶于 950 mL OPA稀釋液(Pickering OD104)中

反應(yīng)器： 45 °C, 0.15 mL

衍生試劑流速： 0.3 mL/min

檢測(cè)器：熒光檢測(cè)器

λex= 330 nm, λem=465 nm

相關(guān)產(chǎn)品目錄

0352-0021	30-MIN HIGH EFFICIENCY OXIDIZED FEED HYDROLYSATE KIT:
1154110T	30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture
1700-3102	Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220	Sodium Diluent, pH 2.20, 4 x 250 mL
Na270	Sodium Eluant, pH 2.80, 4 x 950 mL
Na425	Sodium Eluant, pH 4.25, 4 x 950 mL
Na640	Sodium Eluant, pH 6.40, 4 x 950 mL
RG011	Sodium Column Regenerant, 950 mL
T200	TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
1700-0155	Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 μmole/mL, 5 mL
0352-0022	KIT IDENTICAL TO 0352-0021 WITH ITEMS BELOW REPLACING T200
OD104	OPA Diluent, 4 x 950 mL
O120	o- Phthalaldehyde, 5 g
3700-2000	Thiofluor?, 10 g (2 each per kit)
55 分鐘高效分析包
0352-0031	HIGH-EFFICIENCY 55-MIN PROTEIN HYDROYSIS KIT:
1154150T	Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102	Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220	Sodium Diluent, pH 2.20, 4 x 250 mL
Na315	Sodium Eluant, pH 3.15, 4 x 950 mL
Na740	Sodium Eluant, pH 7.40, 4 x 950 mL
RG011	Sodium Column Regenerant, 950 mL
T200	TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H	Sodium Calibration Standard, for protein hydrolysate, 0.25 μmole/mL, 5 mL
AO31FH	KIT IDENTICAL TO 0352-0031 WITH ITEMS BELOW REPLACING A T200
OD104	OPA Diluent, 4 x 950 mL
O120	o- Phthalaldehyde, 5 g
3700-2000	Thiofluor?, 10 g (2 each per kit)
0352-0032	HIGH-EFFICIENCY 55-MIN COLLAGEN HYDROLYSATE KIT
1154150T	Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102	Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220	Sodium Diluent, pH 2.20, 4 x 250 mL
Na315	Sodium Eluant, pH 3.15, 4 x 950 mL
Na740	Sodium Eluant, pH 7.40, 4 x 950 mL
RG011	Sodium Column Regenerant, 950 mL
T200	TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506C	Sodium Calibration Standard, for collagen hydrolysate 0.25 μmole/mL, Proline and Hydroxyproline, 1.25 μmole/mL, 5 mL
AO32FC	KIT IDENTICAL TO 0352-0032 WITH ITEMS BELOW REPLACING A T200
OD104	OPA Diluent, 4 x 950 mL
O120	o- Phthalaldehyde, 5 g
3700-2000	Thiofluor?, 10 g (2 each per kit)

0352-0017	60-MIN OXIDIZED FEED HYDROLYSATE KIT:
1154150T	Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102	Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220	Sodium Diluent, pH 2.20, 4 x 250 mL
Na270	Sodium Eluant, pH 2.80, 4 x 950 mL
Na740	Sodium Eluant, pH 7.40, 4 x 950 mL
RG011	Sodium Column Regenerant, 950 mL
T200	TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
1700-0155	Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 μmole/mL, 5 mL
0352-0019	KIT IDENTICAL TO 0352-0017 WITH ITEMS BELOW REPLACING T200
OD104	OPA Diluent, 4 x 950 mL
O120	o-Phthalaldehyde, 5 g
3700-2000	Thiofluor?, 10 g, (2 each per kit)
60 分鐘標(biāo)準(zhǔn)分析包
0352-0030	STANDARD 60-MIN PROTEIN HYDROLYSATE KIT
1193250	Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)
1700-3102	Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220	Sodium Diluent, pH 2.20, 4 x 250 mL
Na328	Sodium Eluant, pH 3.28, 4 x 950 mL
Na740	Sodium Eluant, pH 7.40, 4 x 950 mL
RG011	Sodium Column Regenerant, 950 mL
T200	TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H	Sodium Calibration Standard, for protein hydrolysate, 0.25 μmole/mL, 5 mL
AO30SH	KIT IDENTICAL TO 0352-0030 WITH ITEMS BELOW REPLACING T100C
OD104	OPA Diluent, 4 x 950 mL
O120	o-Phthalaldehyde, 5 g
3700-2000	Thiofluor?, 10 g, (2 each per kit)
標(biāo)準(zhǔn)品&測(cè)試品
012506C	Collagen Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
L,L & allo-Hydroxylysine 4-trans-L-Hydroxyproline(1.25)
012506H	Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
Leucine Lysine
1700-0155	Oxidized Feed Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
Leucine Lysine
1700-0070	Amino Acid Test Mixture Concentration 0.25 μmole/mL in 0.01 NHCl, 5.0 mL
Cysteic Acid Threonine Serine

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